To 200 mls of spring water in a beaker add:
0.2 gm Sodium Acetate (Na acetate 3H2O)
0.4 gm Tryptone
0.2 gm Yeast extract
Bring to a boil on the hot plate. When cool pour into a Erlenmeyer flask. Label
flask with date and type of media. Cover the flask with parafilm. To use, pour into
culture tubes or petri dishes and inoculate with protists.